ACE HILIC Method Development Kit

For Developing Reproducible UHPLC / HPLC HILIC Separations

Hydrophilic Interaction Liquid Chromatography (HILIC) uses similar eluents to Reversed-Phase Liquid Chromatography (RPLC) and provides a suitable retention and separation alternative for polar to very polar compounds.  The retention of these hydrophilic analytes (i.e. those with a log P value (octanol-water partition coefficient) of around zero or less) can be a challenge in RPLC characterized by little or no retention.


The ACE HILIC MDK provides 3 HILIC column chemistries for polar retention specifically designed to provide alternative selectivity to each other in HILIC mode.  The chemistries include:


  • ACE HILIC-A (phase with acidic character) 
  • ACE HILIC-B (phase with basic character) 
  • ACE HILIC-N (phase with neutral character)

ACE HILIC / Method Development Kit / ACE-Hichrom

    • Hydrophilic Interaction Liquid Chromatography (HILIC) was first described by Alpert*
    • HILIC is ideal for the separation and retention of polar species including polar neutral and polar ionized analytes
    • HILIC separations typically include a polar stationary phase with a high organic solvent containing mobile phases 
    • Mechanistically HILIC is complex (see Fig) and provides multiple modes of interaction between the analyte, stationary phase, eluent and water enriched layer at the stationary phase particle-eluent interface.
      * A. J. Alpert, J. Chromatogr., 499 (1990) 17